Fig. 1

Summary of liquid chromatography/mass spectrometry (LC/MS) and proteomic analyses of tissue from the cerebrovasculature isolated from the inferior frontal gyrus in Alzheimer’s disease (AD) and healthy control cases from different APOE genotypes. (A) shows brain region of interest used to isolate cerebrovessel fractions. (B) shows identified total number of quantified spectra, peptide spectrum matches and non-redundant master protein groups from all TMT experiments. (C) Data shows level of expression for gene IDs associated with specific cell types identified in our proteomic analyses of the isolated cerebrovasculature (i.e. astrocytes, microglia, pericytes, endothelial cells, and smooth muscle cells). Data represent ratio expressed in arbitrary units. Venn diagram shows unique and overlapping significantly regulated proteins in the comparisons between (D) healthy controls from APOE2/2 vs APOE3/3, APOE3/4 vs APOE3/3, and APOE4/4 vs APOE3/3 genotypes, (E) Healthy matched Alzheimer’s disease cases vs controls from APOE2/3, APOE3/3, APOE3/4, and APOE4/4 genotypes. Asterisk in Venn diagram [*] denote unique non-overlapping proteins from each comparisons. F, G and H shows the violin plot for Amyloid plaque score, Tangle score and CAA score, respectively