Fig. 2

Differentially expressed proteins and cell type origin in healthy controls with  different APOE genotypes. Heat map (Log2FC) of all master proteins identified across the three different genotypes of interests compared to APOE3/E3 controls (A). Volcano plot of differentially expressed proteins in healthy controls from APOE2/E2 vs APOE3/E3 genotypes (B), APOE4/E4 vs APOE3/E3 genotypes (C), and APOE3/E4 vs APOE3/E3 genotypes (D). Pie chart inset of graphs shows up/down-regulated proteins from each comparisons. Pie Chart shows origin of cell types where significant (cerebrovascular cell specific) proteins are observed  between APOE2/E2 vs APOE3/E3 controls (b), APOE4/E4 vs APOE3/E3 controls (c), and APOE3/E4 vs APOE3/E3 controls (d). Values are generated from the ratio of significantly altered cerebrovascular cell specific proteins identified within our entire control datasets, and further expressed as a percentage of all 4 cerebrovascular cell types. EnD—endothelial cells, Ast—astrocytes, Per—Pericytes, SMC—smooth muscle cells. (E) shows heat map of the top 3 altered pathways  from the three different APOE genotype comparisons and the corresponding number of significant proteins and their Log2 fold change expression level