Fig. 5

Identification of a new Homer2 variant, Homer2e, induced by ischemia in mouse cerebellum and by OGD in CGCs. A Upper, nested PCR of mouse cerebellum cDNA performed on the coding region of Homer2a. The first and the second PCR reactions are depicted as 1 and 2. In the second PCR, a small size band just underneath the major band was detected particularly in ischemic and OGD samples (arrow). Lower, RT-PCR detection of Homer2e in the cerebellum and CGCs. Homer2e increased in both ischemic cerebellum and OGD CGCs. Three individual animals (n = 3) and cultures (n = 3) were analyzed in each condition. B Comparison of the partial nucleotide and deduced amino acid sequences between Homer2a and Homer2e. C Comparison of exons between Homer2a/b and Homer2e. Exons 5 and 7–9 of Homer2a/b are lacking in Homer2e. Because the nucleotide deficits cause a frameshift, Homer2e encodes a potential protein of 174 amino acids, which lacks the C-terminal of Homer2a/b