Fig. 3

Comparisons of predicted epigenetic age using the Fetal Brain Clock (FBC) between cellular models throughout differentiation states. A Boxplots comparing the distribution of predicted epigenetic age (days post-conception) in iPSCs and their derived NPCs and neurons, where each panel represents a different dataset. P values of Tukey HSD corrected ANOVA for the Imm dataset and two-sample t-tests for Price, Nazor, Fernández-Santiago and Sultanov datasets are given. F. -S. = Fernández-Santiago. B Boxplots of predicted epigenetic age calculated using the FBC where samples from the five iPSC-Neuron datasets are grouped by cell stage (n = 82, 30 iPSCs, 4 NPCs, 48 iPSC-neurons) and colored by dataset. P values from mixed effects model are given for differences between iPSCs and NPCs (non-significant) and iPSC and neurons. C Boxplots comparing the predicted epigenetic age in a cohort with iPSCs and derived motor neurons. P values of two-sample t-test are given. D Boxplots of predicted epigenetic age by FBC applied on two datasets including ESCs and their derived NPCs and neurons. P values of Tukey HSD corrected ANOVA for the Kim dataset and two-sample t-tests for the Nazor dataset are given