Fig. 1
Hv1 is functionally expressed in spinal microglia and upregulated after SNT. A Representative images of an acute spinal cord slice with a patched GFP-expressing microglia (boxed area was enlarged, upper). Scale bar: 200 µm (left), 40 µm (right). Outward proton currents induced by voltage steps in spinal cord microglia (lower). Increased pHi decreased the outward proton current and shifted the activation threshold to more depolarized potentials in WT microglia. No outward current was observed in Hv1 KO microglia. B I/V curves showing that Hv1 KO abolished voltage-gated proton currents in spinal cord microglia (n = 6 cells from 3 mice each group). C, D Representative Western blot images (C) and quantification data (D) showing that Hv1 expression in the L4-5 level of the dorsal horn increased at POD1-7 after SNT. Data are presented as mean ± SEM, n = 4–5 mice/group. **p < 0.01, ***p < 0.001, GAPDH was used as internal control. Spinal cord tissue from Hv1 KO animals was used as a negative control to confirm the antibody specificity, one-way ANOVA with multi comparisons