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Fig. 3 | Molecular Brain

Fig. 3

From: The voltage-gated proton channel Hv1 promotes microglia-astrocyte communication and neuropathic pain after peripheral nerve injury

Fig. 3

Hv1 deficiency does not alter microglial activation after SNT. A Representative images of Iba1 (top panels), BrdU (middle panels) and merged (bottom panels) immunoreactivity in the ipsilateral dorsal horn in control (left), SNT-induced WT (center) and SNT-induced Hv1 KO (right) mice at POD7. Inserts are higher magnification of boxed regions in the representative low magnification images. B Representative images of Iba1 (top panels), phosphorylated p38 (p-p38, middle panels) and merged (bottom panels) immunoreactivity in the ipsilateral dorsal horn in control (left), SNT-induced WT (center) and SNT-induced Hv1 KO (right) mice at POD3. Inserts are higher magnification of boxed regions. Scale bar:100 µm. C Quantitative summary of microglial density in the contralateral and ipsilateral dorsal horn as well as BrdU + microglia density in the ipsilateral dorsal horn at POD7 following SNT in WT and Hv1 KO mice (n = 10 slices from 3 mice for each group, unpaired t-test). D Quantitative p-p38 immunoreactivity in the contralateral and ipsilateral dorsal horn of WT and Hv1 KO mice. Data are presented as mean ± SEM, cells were randomly picked from 6 slices for each group. WT vs. Hv1 KO., unpaired t-test

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