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Fig. 4 | Molecular Brain

Fig. 4

From: The voltage-gated proton channel Hv1 promotes microglia-astrocyte communication and neuropathic pain after peripheral nerve injury

Fig. 4

SNT-induced ROS production is attenuated in Hv1 KO mice. A Representative images of 8-OHG (top) and merged 8-OHG/Iba1 (bottom) immunostaining in WT (left) and Hv1 KO (right) spinal cord at POD7 after SNT. B Representative images of ox-DHE (top) and merged ox-DHE/CX3CR1-GFP (bottom) signals in WT (left) and Hv1 KO (right) spinal cord at POD3 after SNT. C Representative images of Gp91phox (top) and merged Gp91phox/Iba1 (bottom) immunostaining in WT (left) and Hv1KO (right) spinal cord at POD1 after SNT. Scale bar: 50 µm. D–F Quantitative data showing 8-OHG, ox-DHE and Gp91phox signal in the ipsilateral dorsal horn after SNT. Data are presented as mean ± SEM, 7–15 slices from 3 mice for each group. *p < 0.05; **p < 0.01; ***p < 0.001. WT vs. Hv1 KO unpaired t-test. G Mechanical allodynia in WT and Hv1 KO mice treated with and without ROS scavenger, sulforaphane (SF), 1 h after SNT (arrow). SF treatment significantly attenuated mechanical allodynia in WT mice when compared to control group (WT + saline) but not in H1KO mice with SF or saline treatment. Data are presented as mean ± SEM, n = 7 mice for each group. **p < 0.01; ***p < 0.001, unpaired t-test

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