Fig. 5

Reduced astrocyte activation in Hv1 KO spinal cord after SNT. A Representative images of Iba1 (top panels), GFAP (middle panels) and merged (bottom panels) immunoreactivity in dorsal horn of control (the contra side of a WT sample was shown, left), the ipsilateral of SNT-induced WT (left center), SNT-induced Hv1 KO (right center), and sulforaphane-treated SNT-induced WT (right) mice at POD 7. Inserts are higher magnification of boxed regions. Scale bar: 100 µm. B–D Sample dorsal horn slice (B) is labelled with GFAP-astrocytes (green) and Iba1-microglia (red) highlighting the different lamina of the spinal cord. Astrocytes (C) and microglia (D) density were quantified in WT and Hv1 KO tissues at POD 7. Scale bar: 100 µm. Data are presented as mean ± SEM, images obtained from 3 mice for each group. **p < 0.01; ***p < 0.001, unpaired t-test