Fig. 1
From: Disrupting the α7nAChR–NR2A protein complex exerts antidepressant-like effects
Three i.c.v. injections of TAT-α7-peptide reduces FST immobility time without altering locomotor activity. A The immobility time of animals that received three injections (4 nmol of TAT or TAT-α7-peptide i.c.v., and 20 mg/kg imipramine i.p.) in the FST. The drug was injected immediately after pre-test, 5 h before actual swim test and 1 h before actual swim test. FST was performed 1 h after the last injection and the test was video recorded. Immobility time for each animal was recorded manually. Data are presented as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post hoc test. ns no statistical significance, *p < 0.05, ***p < 0.001 as compared to TAT group. n = 12 (TAT)/14 (TAT-α7-peptide)/7 (imipramine). B Locomotor activity of animals that received three injections (4 nmol of TAT or TAT-α7-peptide i.c.v., and 20 mg/kg imipramine i.p.). The drug was injected 24 h, 5 h and 1 h before the locomotor test. Locomotor activity was measured after 1 h of the last injection. The locomotor activity was measured by total beam breaks in a 1-h time interval. Data are shown as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post hoc test. ns no statistical significance. n = 12 (TAT)/14 (TAT-α7-peptide)/7 (imipramine). C The immobility time of animals that received a single injection (4 nmol of TAT or TAT-α7-peptide i.c.v., saline i.c.v., and 20 mg/kg imipramine i.p.) in the FST. FST was performed 1 h after the last injection and the test was video recorded. Immobility time for each animal was recorded manually. Data are presented as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. ns no statistical significance as compared to saline group, **p < 0.01, ***p < 0.001 as compared to TAT group. n = 6 (Saline)/7 (TAT/TAT-α7-peptide). D Locomotor activity of animals that received a single injection (4 nmol of TAT or TAT-α7-peptide ICV, and saline ICV). The drug was injected 1 h before testing locomotor activity, which was measured by total beam breaks in a 1-h time interval. Data are presented as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post hoc test. ns no statistical significance. n = 6 (Saline)/7 (TAT/TAT-α7-peptide)