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Fig. 1 | Molecular Brain

Fig. 1

From: Y-27632, a ROCK inhibitor, improved laser-induced shock wave (LISW)-induced cochlear synaptopathy in mice

Fig. 1

Changes in the expression levels of ROCKs before and after cochlear damage induced by irradiation of the organ of Corti with laser-induced shock wave (LISW). (ad, a’–d’, a”–d”) Projection images of a confocal series of immunohistochemistry: tissues of the cochlear organ of Corti from the middle turn (16 kHz area) of the mouse before and 1 day after LISW exposure. Immunohistochemistry for ROCK1 (green, anti-ROCK1 (a, b), anti-ROCK2 (c, d)) and merged images with F-actin (rhodamine phalloidin, red) and neurofilament (NF-200, purple) are shown in a’-d’. (a”–d”) Enlarged images of the white inlets in (a’–d’) focusing on the inner hair cells and the end of the peripheral axons of the auditory nerve. After LISW exposure, ROCK1 expression showed ubiquitous elevation around the nerve end (b”), whereas ROCK2 expression showed punctiform enhancement around the inner hair cells (d”, white arrowheads) in addition to the ubiquitous elevation of expression. e The relative mRNA expression levels of RhoA, ROCK1, and ROCK2 before and 1 day after LISW exposure. The relative mRNA expression levels were standardized by the expression levels before LISW exposure in each mRNA (n = 6). f The schema of Rho/ROCK and associated signal pathway. *indicates a significant difference (p < 0.05, two-tailed Mann–Whitney U test). The data are shown as the mean ± standard errors of mean. CRMP-2 collapsing response mediator protein-2, GAP GTPase-activating protein, GEF guanine nucleotide-exchange factor, IHC inner hair cell, LIMK LIM kinase, MLC myosin light chain, OHC outer hair cell, Trk tropomyosin receptor kinase. Scale bar: 20 µm.

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