Fig. 3

The characteristics of the ooG and oG in mouse. a Design of scAAV helper viruses express various RV G genes (ooG and oG). b The workflow of viruses labeling and sample treating. c The specificity of the antibody against the ooG and the oG was determined by western blot. d The ooG and G protein expression in mice brains were measured by Western blotting using antibody against the ooG. e The mRNA copies of the ooG and oG in mice brains were examined using quantitative RT-PCR. The t-test was conducted to compare the difference of mRNA copies of the ooG and oG. “***” Represents a statistically significant difference. The p-value of the ooG versus oG is 0.0004. The data is from the three independent experiments