Skip to main content
Fig. 1 | Molecular Brain

Fig. 1

From: Disruption of the astrocyte–neuron interaction is responsible for the impairments in learning and memory in 5XFAD mice: an Alzheimer’s disease animal model

Fig. 1

Astrocytes’ morphological dynamics and astrocyte–neuron interaction changes during memory formation in the hippocampal DG. a GFAP immunofluorescence in WT and 5XFAD mice after CFC; Z-projection depth is 0–30 μm (scale bar = 50 μm). b Gradation from red to blue in the 3D-reconstructed image; 2 × digital zoom from the 400 × original image, concentric circles are spaced at 10 μm (scale bar = 50 μm). c Quantification of the number of intersections between processes and each circle in the hippocampal DG; WT—Cont (N = 6), 1-h (N = 6), and 24-h (N = 6) groups. d Quantification of the number of intersections between processes and each circle in the hippocampal DG; 5XFAD—Cont (N = 9), 1-h (N = 10), and 24-h (N = 10) groups. e Immunofluorescence for PSD-95 and GFAP in hippocampal DG brain slices of WT and 5XFAD mice; Cont, 1-h, and 24-h groups (scale bar = 20 μm). f Reconstructed images showing a cross-section along the X–Z axis from a confocal Z-stack image from a WT and 5XFAD mouse; Cont, 1-h, and 24-h groups (scale bar = 20 μm). g Quantification of the colocalization of PSD-95 and GFAP in reconstructed cross-section images in the hippocampal DG; WT — Cont (N = 3), 1-h (N = 3), and 24-h (N = 3), 5XFAD—Cont (N = 3), 1-h (N = 3), and 24-h (N = 3) groups. h Immunofluorescence for PSD-95 and GFAP in the hippocampal DG of WT and 5XFAD mice that were treated with vehicle or Stattic (scale bar = 10 μm). i Reconstructed images showing a cross-section along the X–Z axis from a confocal Z-stack image from WT and 5XFAD mice that were treated with vehicle or Stattic (scale bar = 10 μm). j Quantification of the colocalization of PSD-95 and GFAP in the reconstructed cross-section images the hippocampal DG; WT-V (N = 4), WT-S (N = 4), 5XFAD-V (N = 4), 5XFAD-S (N = 4) groups. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with Fisher’s LSD post hoc analysis. Data are presented as mean ± SEM

Back to article page