Fig. 2

Immunofluorescence detection of Bmal1 expression in the hippocampal CA1 and DG of epileptic mice. A Detection of the endogenous Bmal1 protein (red) in CA1 by immunofluorescent labeling. Neurons were labeled by the neuronal marker, NeuN (green). Scale bar, 100 μm. B Analysis of fluorescence intensity was performed using ImageJ. Differences in the relative fluorescence intensity (Bmal1 vs. NeuN) were analyzed with the Students t-test (Control: 1.025 ± 0.015, 60 days: 0.805 ± 0.025, n = 3, p < 0.0001). C Detection of Bmal1 protein (red) and NeuN (green) in DG by immunofluorescent labeling. Scale bar, 100 μm. D Analysis of fluorescence intensity was performed using ImageJ. Differences in the relative fluorescence intensity (Bmal1 vs. NeuN) were analyzed with the Students t-test (Control: 0.926 ± 0.022, 60 days: 0.755 ± 0.047, n = 3, p = 0.008). The data are expressed as mean ± SEM and analyzed with unpaired Student’s t-test. **p < 0.01, ***p < 0.001