Fig. 3
Conditional knockout (cKO) of Bmal1 in DG neurons increased the susceptibility to seizures. A Schematic diagram of AAV injection and the expression of mCherry encoded by an AAV carrying mCherry in DG neurons. Scale bar, 100 μm. B Seizure behavior assessment in control group (n = 10) and cKO group (n = 10) after pilocarpine administration. Data of latency (control: 49.20 ± 3.193; cKO: 37.50 ± 3.583, p = 0.025) are presented as mean ± SEM and analyzed using unpaired Student’s t-test. C Data of mortality rate in the control group and cKO group (n = 10, p = 0.094) were carried out using Fisher’s exact test. D The efficiency of Bmal1 cKO in Bmal1flox/flox mice verified by immunoblotting. Bmal1 expression was significantly decreased at the cKO group, compared with the Ctrl group. Differences in the levels of Bmal1 are presented as mean ± SEM and analyzed using unpaired Student’s t-test (control: 1.000 ± 0.095, 0.270 ± 0.052, n = 3, p = 0.003). E Cre expression was significantly increased at the cKO group, compared with the Ctrl group. Differences in the levels of Cre are presented as mean ± SEM and analyzed using unpaired Student’s t-test (control: 0, cKO: 1.257 ± 0.131, n = 3, p = 0.0007). *p < 0.05, **p < 0.01, ***p < 0.001