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Fig. 2 | Molecular Brain

Fig. 2

From: Meningitic Escherichia coli α-hemolysin aggravates blood–brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling

Fig. 2

Meningitic E. coli infection specifically suppressed TGFBRII expression in BMECs via attenuated Sp1. A The expression of TGFBRII on BMECs of the mice in response to RS218 infection via IF. The BMECs were marked with CD31 in green. Scale bars indicated 50 μm. B, C Expression alteration of TGFBRII (B) or Sp1 (C) in hBMECs along with RS218 infection at indicated time points. **p < 0.01. D The effect of Sp1 overexpression on the RS218 infection-caused TGFBRII reduction in hBMECs by qPCR and Western blot. The Sp1 was cloned in pcDNA3.1(+) and transfected into hBMECs for the overexpression (left panel). **p < 0.01. The qPCR assays were performed in triplicates, and results were presented as mean ± SEM

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Molecular Brain

ISSN: 1756-6606