Skip to main content
Fig. 5 | Molecular Brain

Fig. 5

From: Meningitic Escherichia coli α-hemolysin aggravates blood–brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling

Fig. 5

High-throughput screening of Tn5-transposon mutation library identified α-hemolysin that regulating Gli2 transcription in hBMECs. A Firefly-luciferase reporter assay testing the effect of RS218 infection on the gli2 promoter activity. E. coli K12 strain MG1655 was compared as the control. Results were obtained from three independent assays and presented as mean ± SEM. **p < 0.01. B Schematic diagram showing the screening process of Tn5-transposon mutation library in RS218. C Hemolysis rings of RS218-WT, RS218-ΔhlyA, and RS218-ΔhlyA-phlyCA growing on sheep blood agar plates

Back to article page