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Fig. 1 | Molecular Brain

Fig. 1

From: Cirbp-PSD95 axis protects against hypobaric hypoxia-induced aberrant morphology of hippocampal dendritic spines and cognitive deficits

Fig. 1

Hypobaric hypoxic exposure impaired memory ability and spine morphology. A The scheme of the experimental procedure. B, C Representative locomotion tracking plots (B) and the escape latency (C) of mice in MWM test (n = 8, Student’s t-test, ± SEM). D The latency time prior to descent from the platform in SIAT test (n = 8, Student’s t-test, ± SEM). EI Representative locomotion tracking plots (E), the total distance (F), mean velocity (G), exploring time on new objects (H) and discriminate index (I) of mice in NORT test following normoxia or hypoxia exposure (n = 8, Student’s t-test, ± SEM). J Left: Representative Golgi staining of mice hippocampus. DG, dentate gyrus, scale bar = 50 μm. Middle: Golgi staining of CA1 pyramidal neurons, scale bar = 20 μm. Right: Imaris reconstruction of CA1 pyramidal neurons. K Number of dendritic intersections of reconstructed pyramidal neurons by Sholl analysis (three mice per group, Student’s t-test, ± SEM). L Left: Representative Golgi staining of basal dendritic spines in CA1, scale bar = 20 μm (Red frame indicated target area, scale bar = 5 μm). Right: Representative morphology of apical dendritic spines in CA1, scale bar = 5 μm. M Quantification of basal and apical dendritic spines density and columns represent number of spines per 10 μm (6 neurons/3 mice per group, Student’s t-test, ± SEM). N Quantification of basal and apical neck length (6 neurons/3 mice per group, Student’s t-test, ± SEM). n.s. no significant, *p < 0.05, **p < 0.01, ***p < 0.001

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