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Fig. 2 | Molecular Brain

Fig. 2

From: Cirbp-PSD95 axis protects against hypobaric hypoxia-induced aberrant morphology of hippocampal dendritic spines and cognitive deficits

Fig. 2

Hypobaric hypoxia down-regulated PSD95 protein level in mice hippocampus and ectopic expression of PSD95 restored memory function and the spine phenotype caused by hypoxic exposure. A Representative immunoblotting and the quantification analysis of PSD95 (n = 3 biological replicates, Student’s t-test, ± SEM). B The relative mRNA levels of PSD95 in hippocampus following normoxia or hypoxia exposure (n = 3 biological replicates, Student’s t-test, ± SEM). C Schematic representation of the experimental setup. D The fluorescence image of the hippocampus CA1 after stereotacitic injection showing the autofluorescence of AAV-PSD95 (green) and DAPI (blue) and the immunofluorescence of NeuN (red), scale bar = 50 μm. E, F Representative tracking plots (E) and the escape latency (F) of mice under indicated treatment in MWM test (n = 8, two-way ANOVA, ± SEM). G–K Representative locomotion tracking plots (G), exploring time on new objects (H), discriminate index (I), total distance (J) and mean velocity (K) of mice under indicated treatment in NORT test (n = 8, two-way ANOVA, ± SEM). L The latency time of mice under indicated treatment in SIAT test (n = 8, two-way ANOVA, ± SEM). M, N Representative Golgi staining morphology (M, scale bar = 5 μm) and quantitative analysis of density (N) of apical spines in hippocampus CA1 neurons of mice under indicated treatment (6 neurons/3 mice per group, two-way ANOVA, ± SEM). O Representative immunoblotting of PSD95 (n = 3 biological replicates). n.s. no significant, *p < 0.05, **p < 0.01

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