Fig. 6

Cirbp alleviated hypoxia-induced dendritic spine abnormalities in mice hippocampus. A, B Golgi staining of mice hippocampus (A, scale bar = 50 μm) and of CA1 pyramidal neurons (B, scale bar = 20 μm). C Number of intersections of pyramidal neuron reconstruction (three mice per group, two-way ANOVA, ± SEM). D Representative Golgi staining morphology of apical dendritic spines in CA1, scale bar = 20 μm (red frame indicated target dendritic, scale bar = 5 μm). E Representative morphological images of apical dendritic spines in CA1, scale bar = 5 μm. F Quantitative analysis of basal and apical spines density (6 neurons/3 mice per group, two-way ANOVA, ± SEM). G Quantitative analysis of basal and apical neck length (6 neurons/3 mice per group, two-way ANOVA, ± SEM). H A model for key role of Cirbp-PSD95 axis in hypoxia-induced hippocampal neuron dendritic spine abnormality. *p < 0.05