Fig. 4
LTP induced by SST14 does not require NMDAR or mGluR1a. a Summary time plots of EPSP amplitude for experiments with application of SST14 in the presence (open green circle) and absence (filled green circle) of the NMDAR antagonist DL-APV (50 µM), indicating a similar slow onset LTP in both groups (SST14, n = 7 cells, 5 mice; SST14 + DL-APV, n = 8 cells, 6 mice; two-way mixed ANOVA p = 0.781, main effect of time p < 0.0001 with Bonferroni’s multiple comparisons at 30–35 min p = 0.016). b Summary bar graph of EPSP amplitude, showing similar LTP at 30–35 min post application. c Summary time plots of EPSP amplitude for experiments with application of SST14 in the presence (open green circle) and absence (filled green circle) of the mGluR1a antagonist LY367385 (40 µM), showing similar LTP in both groups (SST14, n = 9 cells, 7 mice; SST14 + LY367385, n = 10 cells, 7 mice; two-way mixed ANOVA p = 0.613, main effect of time p < 0.0001 with Bonferroni’s multiple comparisons at 25–30 min p = 0.004, 30–35 min p = 0.005). d Summary bar graph of EPSP amplitude indicating similar LTP at 30–35 min post application. e EPSPs from a representative cell receiving paired-pulse stimulation before and after SST14 application (right) and summary bar graph (left) showing similar paired-pulse ratio before (− 5 to 0 min) and after (30–35 min) SST14 application (n = 16 cells from SST14 groups in (a) and (c); paired t-test p = 0.531). *p < 0.05; **p < 0.01; ns not significant