Fig. 6
SST14 does not affect EPSPs in PV-INs or PCs. a Montage of fluorescence images showing eYFP expression in hippocampal PV-INs from PV-eYFP mouse. b, c Examples of EPSPs (top) and time plots of EPSP amplitude (bottom) from representative PV-INs receiving 5 µM SST14 (b) or vehicle (c). d Summary time plots of EPSPs (normalized to baseline) for all cells, showing that with application of either SST14 (filled green circle) or vehicle (open green circle), EPSPs showed a gradual run-up with no difference between vehicle and SST14 (Vehicle, n = 10 cells and 6 mice; SST14, n = 9 cells and 6 mice; two-way mixed ANOVA p = 0.141, main effect of time p = 0.0001 with Bonferroni’s multiple comparisons at 10–15 min p = 0.029, 15–20 min p = 0.027, 20–25 min p = 0.042, 25–30 min p = 0.021, 30–35 min p = 0.010). e–g Similar data presentation showing lack of effect of SST14 on EPSPs recorded in CA1 PCs. Examples of EPSPs (top) and time plots of EPSP amplitude (bottom) from representative cells receiving SST14 (e) or vehicle (f). Summary time plots of EPSPs for all cells (g), showing that SST14 (filled green square) or vehicle (open green circle) did not affect EPSPs in PCs. Two-way mixed ANOVA p = 0.506. h, i Bar graphs of EPSP amplitude in PV-INs (h) and PCs (i) at 30–35 min after SST14 or vehicle application showing no difference. *p < 0.05; ns not significant