Fig. 8

Overexpression of LIMK1-EGFP in the hippocampus improves LTP in 3-month old APP/PS1 mice. A Input–output curves of fEPSP showing no differences between APP/PS1 mice expressing EGFP and LIMK1-EGFP (APP/PS1 + EGFP n = 5 slices from 5 mice, APP/PS1 + LIMK1-EGFP n = 6 slices from 6 mice; genotype: F_(1,4) = 0.751, p = 0.435; stimulus intensity: F_(8,32) = 55.649, p < 0.001; repeated two-way ANOVA). B Paired-pulse ratio analysis showing no difference between APP/PS1 mice expressing EGFP and LIMK1-EGFP (APP/PS1 + EGFP n = 6 slices from 5 mice, APP/PS1 + LIMK1-EGFP n = 6 slices from 6 mice; genotype: F_(1,6) = 1.514, p = 0.265; inter-pulse interval: F_(7,42) = 21.588, p < 0.001; repeated two-way ANOVA). Scale bars: 0.2 mV/10 ms. C TBS induced higher LTP in APP/PS1 mice expressing LIMK1-EGFP compared to APP/PS1 mice expressing EGFP. D Summary graph showing significantly enhanced LTP of last 10 min in APP/PS1 mice expressing LIMK1-EGFP compared to APP/PS1 mice expressing EGFP (APP/PS1 + EGFP n = 7 slices from 5 mice; APP/PS1 + LIMK1-EGFP n = 7 slices from 5 mice; p < 0.001, two-tailed t-test). Data are presented as mean ± s.e.m. ***p < 0.001, ns = not significant