Fig. 9
Effect of LIMK1-EGFP expression in 3-month old WT mice. A Normal social interaction during stage 2 of the three-chamber social test in WT mice expressing EGFP or LIMK1-EGFP (WT + EGFP n = 9, p < 0.001; WT + LIMK1-EGFP n = 7, p < 0.001; two-tailed paired t-test). B Preference for S2 over S1 during stage 3 of the three-chamber social test in WT mice expressing EGFP or LIMK1-EGFP (WT + EGFP n = 9, p < 0.001; WT + LIMK1-EGFP n = 7, p < 0.001; two-tailed paired t test). C Discrimination scores during stage 3 of three-chamber social test showing no difference in social memory between WT mice expressing EGFP and LIMK1-EGFP (p = 0.783, two-tailed t-test). D Both EGFP and LIMK1-EGFP expressing WT mice showed memory acquisition during trials 1–5 of the five-trial social memory assay (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7; genotype: F(1,6) = 7.086, p = 0.037; trial: F(4,24) = 9.180, p < 0.001; repeated two-way ANOVA for trial 1–5). On trial 6, WT + LIMK1-EGFP mice showed significantly decreased interaction time compared during the presentation of a novel mouse compared to WT + EGFP mice (p = 0.041, two-tailed t-test). E Open field test showing travel distance in WT mice expressing EGFP or LIMK1-EGFP (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7, p = 0.040, two-tailed t-test). F Average travel speed in pen field test (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7, p = 0.040, two-tailed t-test). G Time spent in center arena in open field test (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7, p = 0.360, two-tailed t-test). H Time spent in peripheral arena in open field test (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7, p = 0.259, two-tailed t-test). I Travel distance in elevated plus maze test (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7, p = 0.594, two-tailed t-test). J Time spent in closed arms in elevated plus maze test (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7, p = 0.187, two-tailed t-test). K Time spent in open arms in elevated plus maze test (WT + EGFP n = 10, WT + LIMK1-EGFP n = 7, p = 0.188, two-tailed t-test). Data are presented as mean ± s.e.m. n = number of mice. *p < 0.05, ***p < 0.001, ns = not significant