Cav-1 expression increases in the brain with age. a Brain hemispheres obtained from WT and A53T transgenic (TG) mice at the age of 2 and 8 months were lysed and Western blot was performed with indicated antibodies. Values were derived from three individual mice (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. b Immunohistochemistry for cav-1 at 2 months or 8 months was performed in WT or A53T TG mice. Images were derived from more than three individual mice. Scale bar indicates 50 μm. ST, triatum; DG, dentate gyrus; CX, cortex; SN, substantia nigra. c Brain tissues containing the hippocampus from A53T TG mice at the age of 8 months were immunostained with indicated antibodies and observed under a confocal microscope. Scale bar indicates 20 μm.