Fig. 2

The uptake of α-syn increases in cav-1 overexpressing SH-SY5Y cells and rat primary cortical neurons. a EGFP only (Con) and WT cav-1-EGFP OE SH-SY5Y cells (Cav-1) were cocultured with differentiated α-syn OE SH-SY5Y cells cultured on the insert for 24 h. The samples were fixed and immunostained with α-syn antibody (BD) and observed under a confocal microscope. The intensity of five independent experiments was analyzed. b Rat primary cortical neurons were transfected with EGFP and WT cav-1-EGFP plasmids. After 1 day of transfection, the cells were cocultured with differentiated α-syn OE SH-SY5Y cells cultured on the insert for 24 h. The samples were fixed and immunostained with human α-syn antibody (Abcam) and observed under a confocal microscope. The intensity of four independent experiments was analyzed. Blue indicates DAPI staining. Scale bars indicate 20 μm. *p < 0.05, **p < 0.01, one-way ANOVA (a), unpaired t-test (b)