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Fig. 4 | Molecular Brain

Fig. 4

From: Age-related increase in caveolin-1 expression facilitates cell-to-cell transmission of α-synuclein in neurons

Fig. 4

The phosphorylation of cav-1 is increased by α-syn fibrils and decreased by c-Src inhibitors. a WT cav-1 stable cell line was treated with 1μM α-syn fibrils for 10 min. Before treatment with α-syn fibrils, 10 μM SKI-1 or 0.1 μM saracatinib were pretreated for 30 min. Each lysate was then analyzed using Western blot. The band intensity of three independent experiments was quantified. b The WT cav-1-EGFP cell line was cocultured with α-syn OE SH-SY5Y cell using the dual-chamber system for 24 h in the presence of 10 μM SKI-1 or 0.1 μM saracatinib. The cells were stained with α-syn antibody (BD) and observed using confocal microscopy. Blue indicates DAPI staining. Scale bar indicates 20 μm. The intensity values are obtained from five independent experiments. **p < 0.01, ***p < 0.001, one-way ANOVA (ab)

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