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Fig. 7 | Molecular Brain

Fig. 7

From: Age-related increase in caveolin-1 expression facilitates cell-to-cell transmission of α-synuclein in neurons

Fig. 7

Cav-1 mediates Lewy body-like inclusion body formation. a Schematic diagram showing coculture assay. bc A53T-E, WT cav-1-A53T-E and Y14A cav-1-A53T-E OE SH-SY5Y cells were cocultured with A53T-M OE SH-SY5Y cells for 5 days in the presence of 50 μM RA. Next, the samples were observed under a confocal microscope, and the number of cells containing double fluorescence-labeled puncta was analyzed. Arrows indicate double fluorescence-labeled puncta. In the graphs, green indicates the number of A53T-E cells, containing double fluorescence-labeled puncta, and red indicates the number of A53T-M cells, containing double fluorescence-labeled puncta. The values obtained are from five independent experiments. d Schematic diagram showing the microfluidic chamber assay. e Primary neurons from A53T TG mice were cultured in microfluidic chambers. At 7 DIV, 0.5 μg of α-syn fibrils were added into chamber 1, and lentiviruses of EGFP-only, WT cav-1-EGFP or Y14A cav-1-EGFP were added into chamber 2. Neurons were fixed at 7 days after treatment with α-syn fibrils. The devices were then removed. The cells were immunostained with pSer129 α-syn antibody. f The intensity was analyzed. Values were derived from four independent experiments. *P < 0.05, **p < 0.01, ***P < 0.001, one-way ANOVA. Blue indicates DAPI staining. Scale bars indicate 20 μm

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