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Fig. 1 | Molecular Brain

Fig. 1

From: Altered hippocampal transcriptome dynamics following sleep deprivation

Fig. 1

Acute sleep deprivation causes substantial gene expression changes in the mouse hippocampus. a Schematic showing experimental procedure for RNA sequencing following acute sleep deprivation. C57BL/6 J male mice were either sleep deprived for 5 h (n = 9) or left undisturbed (n = 9). Immediately following sleep deprivation or undisturbed sleep, the whole hippocampus was dissected out and flash frozen. Total RNA was extracted and processed for RNA sequencing. b Volcano plot illustrating differentially expressed genes between non-sleep deprived and sleep deprived mice. Genes with a false discovery rate (FDR) < 0.1 are highlighted in red for significantly upregulated (507 genes) and blue for significantly downregulated (639 genes) after sleep deprivation. Genes that are not significantly differentially expressed in sleep deprived mice are in grey. c Heatmap showing the most differentially expressed genes filtered by FDR ≤ 0.01 and effect size >  ± 0.5 in each cohort. The top rows represent genes that are significantly downregulated after sleep deprivation. The bottom rows represent genes that are significantly upregulated after sleep deprivation. Each column represents one mouse and columns are grouped by batch. The scale represents log counts per million (logCPM), with red denoting upregulation and blue denoting downregulation after sleep deprivation. The most significantly upregulated gene after sleep deprivation was UPF2 Regulator of Nonsense Mediated MRNA Decay (Upf2; log fold change (LogFC) = 0.263, FDR = 5.01 × 10–6). The most significantly downregulated gene after sleep deprivation was Cold Inducible RNA Binding Protein (Cirbp; LogFC = − 0.516, FDR = 4.83 × 10–6)

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