Fig. 6

Acute sleep deprivation causes distinct transcriptome and translatome patterns. a We compared the top RNA-seq genes (1,146 genes) to our previously generated TRAP-Seq sleep deprivation results (265 genes) and identified 1,035 genes only identified as differentially expressed using RNA-seq (transcriptome), 154 genes only identified as differentially expressed using TRAP-Seq (translatome), and 111 genes found in both. b Using Network Analyst software and the Gene Ontology (GO): Molecular Function (MF) classification, pathways enriched for genes found using both RNA-seq and TRAP-Seq. The most significant pathway was Protein kinase inhibitor activity (P-value = 2.01 × 10^–3, Adjusted P-value = 0.366) and this network has been expanded to show the genes that are transcribed and translated after sleep deprivation. c Using Network Analyst software and the GO:MF classification, pathways enriched for genes found using only RNA sequencing. The most significant pathway was Rho guanyl nucleotide exchange factor activity (P-value = 3.07 × 10^–4, Adjusted P-value = 0.119) and this network has been expanded to show the genes that are involved and transcribed after sleep deprivation. d Using Network Analyst software and the GO:MF classification, pathways enriched for genes found using only TRAP-Seq. The most significant pathway was Unfolded protein binding (Adjusted P-value = 0.021) and this network has been expanded to show the genes that are involved and translated after sleep deprivation. Networks that survive correction for multiple testing (Adjusted P-value < 0.05) are emphasized with dark pink. The size of each node represents the number of hits from the inputted gene list