Fig. 4

Prevention of excitotoxic death with ST2-104. SH-SHY5Y cells were treated with 20 mM glutamate plus 20 μM glycine or control medium or with ST2-104 peptide for 24 or 48 h at 37 °C and then cell viability was evaluated with the MTT assay. Cell viability was measured with the indicated concentrations of ST2-104 peptide for 24 h (a) and 48 h (b). (c), (d) Cell viability was measured with indicated concentration of Glu treatment for 24 h (c) and 48 h (d). e Cell viability was measured with indicated concentration of ST2-104 peptide in Glu-triggered cells. Average death in each coverslip was counted in three fields. The percentage cell viability of 6 wells is represented as S.E. (error bars) (n = 6 for each condition). f The representative images of the cells after insult and treatment groups; scale bar, 50 μm.^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 vs. Con group; ^##P < 0.01 vs. Glu group with one-way ANOVA with Tukey’s post-hoc test