Fig. 6

ST2-104 peptide ameliorated Glu-induced autophagy in SH-SY5Y cells. SH-SHY5Y cells were treated with 20 mM glutamate plus 20 μM glycine or control medium or with ST2-104 peptide for 24 h at 37 °C and then autophagy levels and autophagy-related proteins were assessed. a Representative images of autophagosomes. Arrowheads indicate autophagosomes marked by MDC staining; scale bar, 50 μm. For each well, at least 5 different fields were examined – a representative is shown here. b Detection of LC3-II and Beclin-1 protein levels (representative blot is shown) using Western blot analysis. Levels of β-actin protein were used as the loading control. Bar represents mean ± SEM from 3 separate wells. ^**P < 0.01 vs. Con group; ^##P < 0.01 vs. Glu group with one-way ANOVA with Tukey’s post-hoc test