Fig. 3

Label-free quantitative proteomics of EGFP-Ataxin-3 84Q transgenic zebrafish treated with vehicle and sodium valproate. A Triplicate analyses of vehicle and valproate treated transgenic zebrafish identified common and unique proteins. B Gene ontology (GO) annotation revealed small differences in the identification of proteins, with more proteins (9%) with hydrolase function, while less proteins (3%) were categorized to have carrier activity. C Ingenuity Pathway Analysis (IPA) predicted activation and inhibition of sirtuin signaling and EIF2 signaling pathways respectively upon valproate treatment of transgenic zebrafish expressing EGFP-Ataxin-3 84Q. Blue indicates IPA predicted inhibition and orange indicates predicted activation of categorised biological function and canonical pathways. D Predicted upregulation and downregulation of proteins associated with the sirtuin signaling pathway. Green indicates downregulation (0.67-fold) and red indicates upregulation (1.5-fold) of proteins in valproate treated EGFP-Ataxin-3 84Q zebrafish compared to the vehicle controls