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Fig. 3 | Molecular Brain

Fig. 3

From: Functional organization of the midbrain periaqueductal gray for regulating aversive memory formation

Fig. 3

A Experimental protocol for inactivation of PAG projection neurons during fear learning. B Inactivation of aPVT projecting dlPAG neurons reduced fear learning. A one way ANOVA revealed significant main effect of optogenetic manipulation [F(2, 21) = 9.87, p = 0.001] and post-hoc test showed that averaged CS-evoked freezing during the Test time-point in the ‘Overlap’ group (n = 8) was significantly lower than that of the ‘GFP’ (n = 9) and ‘Offset’ (n = 7) control groups (**p < 0.01). C, D Inactivation of pPVT (C, [F(2, 21) = 0.13, p = 0.875]; n = 8 for all groups) and CM (D, [F(2, 19) = 0.92, p = 0.417]; Overlap n = 8, Offset n = 7, GFP n = 7) projecting dlPAG neurons had no effect on fear learning. E Inactivation of aPVT projecting dlPAG neurons during auditory CS presentation following learning had no effect on the expression of previously learned fear responses. 2-way ANOVA interaction [F(1, 21) = 0.0009, p = 0.9752], n = 6 for both groups

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