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Table 1 Neuronal subtypes analysis of hiPSC-NSPC-derived neurons after 49 days of differentiation

From: Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

hiPSC neurons NGF Type of cells
Glutamatergic nerve Dopaminergic neuron Serotonergic neuron Cholinergic neuron Glial cells
VGLUT1 positive VGLUT2 positive TH positve TPH2 positve VAChT positve ChAT positve GFAP positive
1210B2  +   +   +++   +   +  +   + 
 +   +++   +   +   +   + 
1201C1  +   +   +++   +   +   +   + 
 +   +++   +   +   +   + 
  1. The evaluation of immunofluorescence images are shown (+++: > 80%; ++: 10–80%; +: 1–10%; –: none)