Skip to main content

Table 1 Neuronal subtypes analysis of hiPSC-NSPC-derived neurons after 49 days of differentiation

From: Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

hiPSC neurons

NGF

Type of cells

Glutamatergic nerve

Dopaminergic neuron

Serotonergic neuron

Cholinergic neuron

Glial cells

VGLUT1 positive

VGLUT2 positive

TH positve

TPH2 positve

VAChT positve

ChAT positve

GFAP positive

1210B2

 + 

 + 

 +++ 

 + 

–

 + 

+ 

 + 

–

 + 

 +++ 

 + 

–

 + 

 + 

 + 

1201C1

 + 

 + 

 +++ 

 + 

–

 + 

 + 

 + 

–

 + 

 +++ 

 + 

–

 + 

 + 

 + 

  1. The evaluation of immunofluorescence images are shown (+++: > 80%; ++: 10–80%; +: 1–10%; –: none)