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Table 1 Methodological specifications of ex vivo field recordings in the Schaffer collateral pathway, using Gq- and Gi-DREADD based astrocyte modulation

From: Side-by-side comparison of the effects of Gq- and Gi-DREADD-mediated astrocyte modulation on intracellular calcium dynamics and synaptic plasticity in the hippocampal CA1

  Current study, Van Den Herrewegen et al. (2021) Adamsky et al. [36] Nam et al. [16]
Viral vectors and used chemogenetic tools
Viral vector with DREADD construct *Gq-DREADD: AAV8-GFAP-hM3Dq-mCherry
*Gi-DREADD: AAV8-GFAP-hM4Di-mCherry
*Control vector: AAV8-GFAP-mCherry
*Gq-DREADD: AAV8-GFAP-hM3Dq-mCherry
*Gi-DREADD: /
*Control vector: AAV8-GFAP-mCherry
*Gq-DREADD: /
*Gi-DREADD: AAV-GFAP-hM4Di-mCherry
*Control vector: AAV-GFAP-GFP
Animals
Mouse strain/age C57Bl6J/11–13 weeks C57Bl6J/11–13 weeks C57Bl6J/7–8 weeks
Gender Male Male Male
Group/single housed Group Group Ns
Slicing conditions
Slice orientation and thickness Coronal
400 µm
Coronal
400 µm
Transverse
350–400 µm
Slicing solution (in mM) 75 Sucrose, 87 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 7 MgCl2, 0.5 CaCl2, 0.6 Ascorbic acid and 25 glucose 126 NaCl, 2.6 KCl, 26 NaHCO3, 1.25 NaH2PO4, 1 MgCl2, 0.625 CaCl2 and 10 glucose 212.7 sucrose, 5 KCl, 26 NaHCO3, 1.23 NaH2PO4, 10 MgSO4, 0.5 CaCl2, and 10 dextrose
Slice recovery conditions 1 h submerged 1 h submerged 1 h ns
DREADD agonist
DREADD agonist CNO CNO CNO
Concentration 10 µM 10 µM ns
Duration of CNO application 25 min 25 min 10 min
Slice electrophysiology recording conditions
Stimulation frequency 0.033 Hz 0.017 Hz 0.07 Hz
Stimulations during baseline 30 min (60 stimulations) 10 min (10 stimulations) 10 min (40 stimulations)
Pause in stimulation Yes Yes No
Stimulations necessary for induction of synaptic potentiation Gq-DREADD: 21
Gi-DREADD: 1
Gq-DREADD: 1 (1 mM Mg2+)
21 (2 mM Mg2+)
Gi-DREADD: 40+ additional 40 Hz stimulus
Bath temperature 28 °C 32 °C 28–30 °C
aCSF composition (in mM) 124 NaCl, 3 KCl, 26 NaHCO3, 1.4 NaH2PO4, 1 MgSO4, 2 CaCl2 and 10 glucose 126 NaCl, 2.6 KCl, 26 NaHCO3, 1.25 NaH2PO4, 1 MgCl2, 2 CaCl2 and 10 glucose 124 NaCl, 5 KCl, 26 NaHCO3, 1.23 NaH2PO4, 1 MgSO4, 2 CaCl2 and 10 dextrose
  1. ns not specified