Fig. 3

EA stimulation at the Baihui (GV 20) and Dazhui (GV 14) acupoints improves RSDS-evoked anxiety-like behaviors in the light–dark box task. A Representative heat maps for the activities of each group during the light–dark test. The frequency spent in the light compartment (B), the latency to the first entry into light compartment (C), or the cumulative time in the light compartment (D) were determined. The velocity traveled in the light compartment was measured in (E). Quantitative data are presented as the mean ± SEM (control: n = 13; RSDS: n = 11; RSDS/Sham: n = 9; RSDS/EA n = 13). One-way ANOVA with a post-hoc Tukey test was used to examine the significance of the mean. *** p < 0.001 vs. the control group. **** p < 0.0001 vs. the control group. ## p < 0.01 vs. the RSDS group. ### p < 0.001 vs. the RSDS group. ANOVA: analysis of variance, EA: electroacupuncture, NS: not significant, RSDS: repeated social defeat stress, SEM: standard error of mean