Fig. 4
From: Ackr3-Venus knock-in mouse lights up brain vasculature
ACKR3-Venus expression in peripheral mouse organs. Representative images of amplified ACKR3-Venus expression from eight peripheral tissues prepared from adult (P56 to P72) female and male mice. Organs from Ackr3Venus/Venus mice were sectioned transversely (4 µm), and the expression of Venus was revealed with a mouse Venus (GFP) antibody (brown) and counterstained with hematoxylin labeling of cell nuclei (blue). Shown are representative images of organs where ACKR3-Venus is observed A heart (male) B stomach (female) C intestine (male) D kidney (female) E Thymus (female) F colon (female) G lung (male) H Fat (male). Arrowheads are located at regions with ACKR3-Venus expression. A red box on the inset indicates where the higher magnification image was taken. Organ sections were imaged on the Hamamatsu’s NanoZoomer® Digital Pathology system 2HT with 80× (large image) and 20× (inset) objectives. Scale bar is 25 µm