Fig. 6
From: Ackr3-Venus knock-in mouse lights up brain vasculature
ACKR3-Venus whole brain distribution in the adult mouse brain. Amplified ACKR3-Venus expression was examined throughout the adult (P56) mouse brain (Table 3). Brains from Ackr3Venus/Venus mice (n = 4) were sectioned coronally on a cryostat (30 µm). Venus fluorescence was amplified with Anti-GFP (red), and slides were scanned on a slide scanner widefield microscope (Olympus). Images were scanned with a 10x objective. Top—Annotated regions shown here in red, contained the highest enrichment of ACKR3-Venus expression. Bottom—A scheme demonstrates the range of observed ACKR3-Venus expression across brain regions. Ctx cortex, OFC orbital-frontal cortex, DG dentate gyrus, HPF hippocampal formation, MB midbrain, Pn pons, Hy Hypothalamus, IC inferior colliculus, MM medial mammillary, STR striatum, SOC superior olivary complex, TH Thalamus, ND nucleus of Darkschewitsch. Scale bar is 1000 µm