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Fig. 7 | Molecular Brain

Fig. 7

From: Ackr3-Venus knock-in mouse lights up brain vasculature

Fig. 7

ACKR3-Venus expression in glial and neuronal cell-types in the adult mouse brain. ACKR3-Venus was examined at the cellular level using cell subtype markers. Shown are representative images for intrinsic (yellow) or amplified anti-Venus (red) with the cell sub-type marker (magenta, if imaged together with intrinsic Venus (A, B, D) or cyan, if imaged with amplified (Amp.) Venus (C, E, F)]. In each row, the first image is 20 × magnification to show brain area and the following three images are at 5 × higher magnification to show cellular level. The middle two images in black and white indicate single antibody channel and the color images are merged images of both channels. A Astrocytes identified by anti-GFAP (magenta) are shown together with intrinsic Venus (yellow) in the molecular layer (ml) of the hippocampus (HPF). B Neurons identified by anti-NeuN (magenta) are shown together with intrinsic Venus (yellow) in the polymorphic layer (po) of the dentate gyrus in the HPF. C Neurons identified by anti-NeuN (cyan) are shown together with amplified Venus (red) in the orbital frontal cortex (OFC). D GABAergic interneurons identified by anti-GABA (magenta) are shown together with intrinsic Venus (yellow) in the po of the dentate gyrus in the HPF. E, F Newly generated neurons identified by anti-DCX (cyan) are shown together with amplified Venus (red) in the olfactory bulb (E) and at the intersection of the granule cell layer (sg) and po of the dentate gyrus in the HPF (F). White boxes (left column) correspond to the 5 × magnified images. Insets contain mouse brain reference atlas images from Allen Brain Atlas, with the region locations outlined by black boxes. White arrowheads indicate cells that are co-labeled, AD or label adjacent, E, F. Brain sections from Ackr3Venus/Venus mice were imaged on an Olympus FV1200 laser scanning confocal microscope, 20 × objective. Scale bar of 20 × image is 100 µm (1st column) and 20 µm (2nd–3rd columns)

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