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Table 3 ACKR3-Venus mapping in the adult mouse brain

From: Ackr3-Venus knock-in mouse lights up brain vasculature

Cortex

Cortical Subplate

Midbrain cont

 Agranular Insular

+ + 

 Bed Nucleus of the stria Terminalis

+ 

 IPN Dorsolateral Subnucleus

+ + 

 Auditory

+ + 

 IPN, Lateral Subnucleus

+ + 

 Cingulate

+ + 

Thalamus

 IPN, rostral Subnucleus

+ + 

 Dysgranular Insular

+ + 

 Anteromedial nucleus

 +  + 

Interstitial nucleus of Cajal

+ + 

 Ectorhinal

+ + 

 Central Medial

+ + 

 Periaqueductal Gray

 Entorhinal

+ + 

 Geniculate

+ + 

  Dorsomedial

+ + 

 Granular Insular

+ + 

 Ethmoid

+ 

  Supraoculomotor

+ + 

 Motor

+ + 

 Interanteromedial

+ + 

  Posterior commissure nucleus

+ 

 Orbital Frontal

+ + + 

 Intergeniculate

+ 

 Pretectal nucleus

+ + 

 Parietal Association

+ + 

 Intermediodorsal

+ + 

 Raphe nucleus

 Piriform

+ + 

 Laterodorsal

+ + 

  Dorsal, Caudal

+ 

 Primary Somatosensory

+ + 

 Lateral Posterior, Mediocaudal

+ 

  Dorsal, Ventral

+ + 

 Secondary Somatosensory

+ + 

 Mediodorsal

+ + 

 Median

+ + 

 Retrosplenial

+ + 

 Medial Geniculate

+ + 

 Substantia Nigra Compact part

+ + 

 Temporal Association

+ + 

 Paracentral

+ + 

 Superior Colliculus

 Visual

+  + 

 Paraventricular

+ 

  Intermediate White layer

+ + 

 Olfactory Bulb

+ + 

 Posterior

+ 

  Optic Nerve layer

+ + 

 Rhomboid

+ + 

Cortical Subplate

 Submedius

+ + 

Pons

 Basal Lateral Amygdala

+ 

 Subparafascicular

+ 

 Dorsomedial Tegmental area

+ 

 Claustrum

+ + + 

 Ventral

+ + 

 Laterodorsal Tegmental nucleus

+ + 

 Epithalamus

 Lateral Lemniscus

+ + + 

Striatum

  Lateral Habenula

+ + 

 Locus Coeruleus

+ 

 Amygdala

  Medial Habenula

+ + 

 Motor Trigeminal nucleus

+ + + 

  Central

 Tensor Tympani part

+ + 

   Lateral

+ + 

Hypothalamus

 Parabrachial nucleus

+ 

   Medial

+ + 

 Arcuate

 + 

 Pontine nuclei

+ + + 

   Extended

+ 

 Dorsomedial

 + 

 Pontine Reticular nucleus

+ 

   Intercalated

+ + 

 Lateral Mammillary

+  +  +  + 

 Posterodorsal Tegmental nucleus

+ + 

   Medial Anterodorsal

+ + 

 Preoptic

+ 

 Principal Sensory Trigeminal nucleus

+ + + 

 Lateral Septum

 Lateral

+ 

 Reticulotegmental nucleus

+ + 

  Dorsal

+  + 

 Median Eminence

+ 

 Superior Olive

+ + + 

  Ventral

+ 

 Paraventricular

+  +  + 

Nucleus Accumbens

+ 

 Perifornical

+ 

Medulla

 Retrochiasmatic

+ 

 Cochlear nucleus

+  +  + 

Hippocampus

 Septohypothalamic

+ 

 Cuneiform nucleus

+ 

 CA2

+ + + + 

 Striohypothalamic

+ 

 Efferent Vestibular nucleus

+ + 

 CA3

+ + + + 

 Subparaventricular

+ 

 Gigantocellular Reticular nucleus

+ + 

 Polymorph layer Dentate Gyrus

+ + + + 

 Suprachiasmatic

+ 

Medial Vestibular nucleus

+ + 

 Fasciola cinereum

+ + + + 

 Supraoptic

+ + + 

Parapyramidal nucleus

+ 

 Fimbria

+ 

 Ventromedial

+ 

Parvicellular Reticular nucleus

+ + 

 Parasubiculum

+ + 

 Postsubiculum

+ + 

Midbrain

Cerebellum

 Presubiculum

+ + 

 Darkschewitsch nucleus

+ + + 

 4th/5th Cerebellar lobules

+ + 

 Pyramidal cell layer

+ + + + 

 Inferior colliculus

+ + + 

 Crus 1 Cerebellar lobules

+ + 

 Interpeduncular Nucleus (IPN)

+ 

 Flocculus

+ + 

  1. Amplified ACKR3-Venus expression was mapped throughout the adult (P56) Ackr3Venus/Venus mouse brain. If ACKR3-Venus expression was not observed in a region or was not observed at the same expression level consistently across animals (n = 4), the region was not included in this table. Expression in Ackr3Venus/Venus (n = 4) were compared to negligible background staining observed in Ackr3WT/WT (n = 4) (Additional file 1: Figure S8). The brain regions included in this table are derived from The Mouse Brain in Stereotaxic Coordinates, Paxinos and Franklin, Second Edition, 2001. The expression of ACKR3-Venus across brain regions is ranked as follows: low (+), moderate (+ +), high (+ + +), very high (+ + + +) expression. This analysis was qualitative, and no statistical analysis has been done