Fig. 4
The genetic defects altered the production and release of neurotransmitters. a Heatmaps displaying the Z-scores of the levels of neurotransmitters, extracted from organoids and media, in HC and dCOs (PRNPE200K1, PRNPE200K2, T21, LRRK2G2019S). The heatmap key is on the right panel. b–e Some of the raw data summarized in a including serine (b), aspartate (c), dopamine (d), and GABA (e). b–e consisted of n = 12 for the HC and n = 4 for the other organoid lines. Levels of neurotransmitters in the organoids with genetic defects were compared to the HC by One-way ANOVA with Dunnett’s correction for multiple comparisons. Each point on the graphs represents an individual organoid. Bars and error denote mean and SEM. * p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001