Fig. 4

Cx43 GJs and HCs role in the increased calcium dynamics in CKs and LPS. A Western blot experiments (left) and analysis (bar plot, right) for the protein Cx43 in Control, CKs and LPS treated slices. Values are expressed as % of Control. **P < 0.01 and *P < 0.05, one-way ANOVA. B Representative high magnification (40 ×) confocal images of EtBr uptake (30 min, in red) in astrocytes (GFAP, in green) induced by CKs and LPS treatments, scale bar 25 µm. EtBr uptake is quantified in the bar plot, normalized to the total number of astrocytes/field, in the absence or in the presence of carbenoxolone (CBX, 200 µM) pre-incubation (30 min). ***P < 0.001, one-way ANOVA