Fig. 1

Identification of cell types from whole-brain cells. a UMAP plot shows the unsupervised clustering of 16,028 cells derived from mouse brain (for details, see “Methods”). The 15 cell types were classified by their transcriptional profiles. b Dot plot shows the expression levels of a representative cell-specific marker gene for each cell type. c UMAP plot shows the distribution of Olig2 expression. d Violin plot shows Olig2 expression across all clusters. e UMAP plot shows the distribution of GFAP expression. f Violin plot shows GFAP expression across all clusters. g A total of 3522 cells of the astrocyte subset were derived from cells in mouse brain. They were classified into Olig2(+)/GFAP(−) astrocytes (347 cells), Olig2(+)/GFAP(+) astrocytes (104 cells), Olig2(−)/GFAP(+) astrocytes (848 cells) and Olig2(−)/GFAP(−) astrocytes (2,223 cells). immNEU immature neuron, mNEU mature neuron, OPC oligodendrocyte precursor cell, immOLG immature oligodendrocyte, OLG oligodendrocyte, ASC astrocyte, OEG olfactory ensheathing glia, MG microglia; MAC macrophage, EC endothelial cell, PC pericyte, EPC ependymal cell, VLMC vascular and leptomeningeal cell, ABC arachnoid barrier cell, CP cycling progenitor