Fig. 3
From: Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
Olig2- and GFAP-astrocytes can be differentially collected from the LGP in Olig2CreER:ROSA-tdTomato mice by laser microdissection. a Experimental schedule of tamoxifen (TAM) administration. b Histological localization of the LGP of the mouse [40]. Numerical values indicate posterior distance (mm) from the plate of Bregma. c Olig2-astrocytes (tdTomato red fluorescence) and GFAP-immunolabeled astrocytes (green fluorescence) show mutually exclusive localization in the LGP. d Schematic representation of the selective dissection of Olig2- and GFAP-astrocytes by laser microdissection. e PCR analysis of selected transcripts in laser-microdissected LGP (top), Olig2-astrocytes (middle) and GFAP-astrocytes (bottom). There is no mutual contamination in the collected samples between Olig2- and GFAP-astrocytes and other cell types. These samples derive from the same experiment and the gels were processed in parallel. Full-length gels are presented in Additional file 1: Fig. S1. f RT-qPCR analysis for mRNA expression levels of astrocyte-specific genes in the two distinct populations of astrocytes. gfap mRNA expression in Olig2-astrocytes was significantly lower than that in GFAP-astrocytes, and there was no significant difference for other pan-astrocytic marker genes. g RT-qPCR analysis for mRNA expression levels of SLC membrane transporter genes. slc7a10 and slc6a13 mRNA expression was significantly higher in Olig2-astrocytes than in GFAP-astrocytes. Graphical data are presented as the mean ± SEM. Student’s t-test was used to compare mean values for unpaired data. Differences were considered significant when **p < 0.01, ***p < 0.001