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Fig. 1 | Molecular Brain

Fig. 1

From: Conditional deletion of ROCK2 induces anxiety-like behaviors and alters dendritic spine density and morphology on CA1 pyramidal neurons

Fig. 1

Generation of ROCK2fl/fl mice. a Schematic representation of the initial allele (tm1a) containing LacZ reporter-promoter driven neo targeting cassette, FLP-FRT sites, loxP sites, and exon 2 that encodes for the kinase domain of ROCK2. The tm1a mice were crossed to B6N.ROSA26:FLPe mice to generate ROCK2 floxed mice (tm1c). Generation of floxed ROCK2 mice allows for CaMKII-Cre-mediated deletion of exon 2. b tdTomato signal in the forebrain of Cre/tdTomato compared to Cre-negative littermates. Scale bar = 2.5 mm. c Representative western blot of ROCK2 and GAPDH levels from hippocampus (HIP) and prefrontal cortex (PFC) of ROCK2fl/fl and Cre/ROCK2fl/fl mice at 8–9 months. d For densitometry analysis protein levels of whole homogenates from ROCK2fl/fl and Cre/ROCK2fl/fl were normalized to GAPDH. Cre/ROCK2fl/fl whole homogenate showed decreased ROCK2 levels in the hippocampus (t(10) = 3.531, **p = 0.0054) and PFC (t(9) = 3.718, **p = 0.0048) compared to ROCK2fl/fl mice. N = 6 ROCK2fl/fl mice (3 M, 3 F) and 6 Cre/ROCK2fl/fl (3 M, 3 F) mice at 8–9 months of age. Unpaired t-tests were used for all comparisons. ROUT outlier test identified one outlier in PFC data set (Cre/ROCK2fl/fl: 1.416336). Error bars represent standard error of the mean. R2fl/fl, ROCK2fl/fl; Cre/R2fl/fl, Cre/ROCK2fl/fl

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Molecular Brain

ISSN: 1756-6606