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Fig. 7 | Molecular Brain

Fig. 7

From: Conditional deletion of ROCK2 induces anxiety-like behaviors and alters dendritic spine density and morphology on CA1 pyramidal neurons

Fig. 7

Medial prefrontal cortex spine density and morphology in Cre/ROCK2fl/fl mice. a Schematic representation of iontophoretic injection sites in layers 2/3 of the prelimbic medial prefrontal cortex (mPFC). b Representative maximum intensity projections of deconvolved confocal z-stacks of Lucifer yellow-filled dendrites in layer 2/3 medial prefrontal cortex are shown (left) for ROCK2fl/fl and Cre/ROCK2fl/fl mice at 8–9 months of age. Corresponding three-dimensional reconstructions of the dendrites are provided (right), with the spines color-coded by spine type (blue = thin, orange = stubby, green = mushroom, yellow = filopodia). Scale bars = 6 µm. c Overall mean spine density and d densities of thin, stubby, and mushroom spines, e spine length, and f head diameter were similar between groups. There were no differences in apical g spine density, h densities of thin, stubby, and mushroom spines, i spine length, and j head diameter. Cre/ROCK2fl/fl mice had no differences in basal k spine density, l densities of thin, stubby, and mushroom spines, m spine length, and n head diameter compared to ROCK2fl/fl littermates. N = 5 ROCK2fl/fl mice (2 M, 3 F) and 7 Cre/ROCK2fl/fl (4 M, 3 F) mice at 8–9 months of age. Unpaired t-tests were used for all comparisons. Each point represents one mouse and the error bars indicate the standard error of the mean. R2fl/fl, ROCK2fl/fl; Cre/R2fl/fl, Cre/ROCK2fl/fl

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