Fig. 3
From: Retrosplenial cortex in spatial memory: focus on immediate early genes mapping
The development of experimental approaches towards detection of IEG engram and their applications in the study of spatial memory. A Immunohistochemistry (or immuofluorescence) detection of FOS protein after behavioral session. B Compartment analysis of temporal activity by fluorescent in-situ hybridization (catFISH) of Arc and Homer mRNA (courtesy of W. Karwicka and A. Hamed). C Co-detection of Fos fluorescent reporter transgene and FOS protein (courtesy of K. Andraka and E. Knapska). D Co-detection of pharmacologically controlled Fos reporter transgene and FOS protein (courtesy of J. Wilmot and B. Wiltgen). E Chronic in vivo imaging of FOS fluorescent IEG reporter