Fig. 3
Dual-color fluorescence time-lapse imaging of the red-DA biosensor R-GenGAR-DA1.2 combined with the green-NE biosensor GRABNE1m in HeLa cells. a Representative image of HeLa cells co-expressing DA1.2 and NE1m. b Top: bars show the schedule of agonist/antagonist application to both DA1.2 and NE1m. Gray vertical lines indicate time of application. Concentrations: DA and SCH, 5 µM; NE and YO, 1 µM. Temperature equilibration and pre-illumination were conducted before compounds application (Additional file 1: Fig. S5d). Bottom: enlarged time-lapse images in the pseudocolor intensity-modulated display mode from the white boxed region shown in panel (a). c The fluorescence intensity change (ΔF/F0) of DA1.2 (top) and NE1m (bottom) in HeLa cells co-expressing DA1.2 and NE1m. Vehicle, 10 µM HCl in H2O or 0.1% DMSO; control, cells were only exposed to excitation light. Mean ΔF/F0 values of 30 cells from 3 experiments are shown with SD (shaded areas). Result of statistical test is shown in Additional file 1: Fig. S11a, b