Fig. 1
From: The exit of axons and glial membrane from the developing Drosophila retina requires integrins
Knocking down mys in both WG and eye-disc epithelium caused a failure of R-cell axons and WG membrane to exit the eye disc. 3rd-instar larval eye–brain complexes were double-stained with MAb24B10 (green) and anti-Bdl (magenta). MAb24B10 (green) labels all R-cells, and anti-Bdl (magenta) labels WG. A–Aʺ The pattern of R-cell axonal projection and WG membrane extension in wild type. A MAb24B10 staining. R-cell axons migrate towards the posterior end of the eye disc (ey) where they converge and enter the optic stalk (os). After exiting the optic stalk, R1–R6 axons terminate at the intermediate target region in the lamina (la), and R7 and R8 axons pass through the lamina into the deeper medulla layer (me). Aʹ The same section stained with anti-Bdl to visualize WG. WG membrane extends from the eye disc through the optic stalk into the distal region of the lamina (arrowheads). MAb24B10 and anti-Bdl staining also visualized Bolwig’s Nerve (BN in A and Aʹ) that projects from Bolwig’s organ in the larval anterior region into the optic lobe long before the birth of R cells and WG. Aʺ The section visualized with both MAb24B10 and anti-Bdl staining. Note that WG membrane associates with R-cell axons in the lamina (arrowheads). B–Bʺ A third-instar eye–brain complex in which UAS-mys-RNAi-JF02819 was simultaneously expressed in both eye-disc epithelium and WG under control of the eye-specific ey3.5-GAL4 and WG-specific Mz97-GAL4 drivers. mys knockdown caused the stalling of R-cell axons (B and Bʺ) and WG membrane (Bʹ and Bʺ) in the eye disc. Note mys knockdown did not affect the projection pattern of pre-existing Bolwig’s Nerve (BN). Scale bar: 20 μm