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Fig. 7 | Molecular Brain

Fig. 7

From: The exit of axons and glial membrane from the developing Drosophila retina requires integrins

Fig. 7

Interfering with the function of Rhea in eye-disc epithelium but not WG also caused the stalling of R-cell axons and WG membrane in the eye disc. 3rd-instar larval eye–brain complexes were double-stained with MAb24B10 (green) and anti-Bdl (magenta). A Wild type. B In an individual in which rhea was knocked down in the eye disc by expressing UAS-rhea-RNAi-HMS00799 (i.e. UAS-rhea-RNAi #1) under control of ey3.5-GAL4, the stalling of R-cell axons and WG membrane in the eye disc and optic stalk was observed. C In an individual in which rhea was knocked down only in eye-disc epithelium by expressing another independent UAS-rhea-RNAi line (i.e. UAS-rhea-RNAi-HMS00856 or UAS-rhea-RNAi #2) under control of ey3.5-GAL4, most R-cell axons and WG membrane stalled in the eye disc. D In many eye-specific mosaic animals in which large clones of homozygous rhea1 mutant eye tissues were generated, R-cell axons and WG membrane failed to exit the eye disc. Scale bar: 20 µm

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